Determination of Spectrophotometric Absorptivity by Analytical Ultracentrifugation
نویسنده
چکیده
Rapid determination of the absorptivity for a recombinant IgG monoclonal antibody using the Beckman equipped with both Raleigh interference and UV absorbance optical systems. The analytical ultracentrifuge data for determining spectrophotometric absorptivities is compared to experimental data from quantitative amino acid analysis and an enzymatic digestion method.
منابع مشابه
A-1815A: Rapid Determination of Spectrophotometric Absortivity by Analytical Ultracentrifugation
Spectrophotometric determination of protein concentrations is an important analytical tool for nearly all phases of protein drug development and manufacturing. Determinations of absorptivities (A0.1%) and molar extinction coefficients (ε) for protein solutions require at least one initial, accurate measurement of protein concentration. Traditionally, this crucial concentration has been determin...
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